Coding

Part:BBa_K1842003:Design

Designed by: Walter Thavarajah   Group: iGEM15_UC_San_Diego   (2015-08-17)


LuxCDEE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 601
    Illegal BglII site found at 2813
    Illegal BglII site found at 4031
    Illegal BamHI site found at 3570
    Illegal BamHI site found at 4788
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3247
    Illegal AgeI site found at 4465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90


Design Notes

The coding sequence was too large to be synthesized in one piece, so it was instead synthesized in a series of fragments that were assembled via Gibson Assembly.


Source

Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum.

References